Category Archives: Calcium Imaging

Fast z-scanning using a voice coil motor

Posted on April 6, 2016 by P.T.R. Rupprecht

We just published a paper on fast remote z-scanning using a voice coil motor. For 2P calcium imaging. It’s a nice paper with some interesting technical details. The starting point was the remote z-scanning scheme used by Botcherby et al. (2012) from … Continue reading →

Posted in Calcium Imaging, Imaging, Microscopy, Neuronal activity | Tagged , , , | 3 Comments

Modulating laser intensity on multiple timescales (x, y and z)

Posted on March 25, 2016 by P.T.R. Rupprecht

In point-scanning microscopy and especially when using resonant scanners, the intensity of the beam is typically modulated using a Pockels cell. For resonant scanning, the dwell time per micrometer is not constant along the scanned line, and one wants either to … Continue reading →

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Undistort/unwarp images for resonant scanning microscopy

Posted on February 16, 2016 by P.T.R. Rupprecht

For image acquisition using a resonant scanning microscope, one of the image axes is scanned non-linearly, following the natural sinusoidal movements of the resonant scanner. This leads to a distortion of the acquired images, unless a online correction algorithm or … Continue reading →

Posted in Calcium Imaging, Microscopy | Tagged , , , , | 1 Comment

Beyond correlation analysis

Posted on February 8, 2014 by P.T.R. Rupprecht

There are many papers out that use calcium imaging of a lot (tens to thousands) of neurons in animal brains. When I first encountered these kind of publications (roughly a year ago), it took me some hours to become familiar … Continue reading →

Posted in Calcium Imaging, Data analysis, Neuronal activity | 2 Comments