Monthly Archives: May 2021

Online spike rate inference with Cascade

To infer spike rates from calcium imaging data for a time point t, knowledge about the calcium signal both before and after time t is required. Our algorithm Cascade (Github) uses by default a window that is symmetric in time … Continue reading

Posted in Calcium Imaging, Data analysis, machine learning, Neuronal activity | Tagged , , , | Leave a comment

Heating up the objective for two-photon imaging

To image neurons in vivo with a large field of view, a large objective is necessary. This big piece of metal and glass is in indirect contact with the brain surface, with only water and maybe a cover slip in … Continue reading

Posted in Calcium Imaging, Imaging, Microscopy | Leave a comment