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Tag Archives: Scanning
Modulating laser intensity on multiple timescales (x, y and z)
In point-scanning microscopy and especially when using resonant scanners, the intensity of the beam is typically modulated using a Pockels cell. For resonant scanning, the dwell time per micrometer is not constant along the scanned line, and one wants either to … Continue reading
Posted in Calcium Imaging, Imaging, Microscopy
Tagged Calcium Imaging, laser scanning microscopy, Microscopy, Scanning
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Undistort/unwarp images for resonant scanning microscopy
For image acquisition using a resonant scanning microscope, one of the image axes is scanned non-linearly, following the natural sinusoidal movements of the resonant scanner. This leads to a distortion of the acquired images, unless a online correction algorithm or … Continue reading
Posted in Calcium Imaging, Microscopy
Tagged Calcium Imaging, laser scanning microscopy, Microscopy, Scanning, zebrafish
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Some interesting 2-photon microscopy papers
In the last few months, I built a special kind of 2P-microsope. In the meantime, I encountered some papers on microscope techniques which I found interesting and worth a side-note. Using AODs instead of galvoscanners for point-scanning: High-speed in vivo … Continue reading
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Tagged Calcium Imaging, Microscopy, Scanning, Temporal Focusing
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