Tag Archives: Scanning

Preamplifier bandwidth & two ways of counting photons

Posted on September 20, 2016 by P.T.R. Rupprecht

For two-photon point scanning microscopy, the excitation laser is typically pulsing at a repetition rate of 80 MHz, that is one pulse each 12.5 ns. To avoid aliasing, it was suggested to synchronize the sampling clock to laser pulses. For this, it is important … Continue reading →

Posted in Calcium Imaging, Imaging, Microscopy | Tagged , , , | 9 Comments

Fast z-scanning using a voice coil motor

Posted on April 6, 2016 by P.T.R. Rupprecht

We just published a paper on fast remote z-scanning using a voice coil motor. For 2P calcium imaging. It’s a nice paper with some interesting technical details. The starting point was the remote z-scanning scheme used by Botcherby et al. (2012) from … Continue reading →

Posted in Calcium Imaging, Imaging, Microscopy, Neuronal activity | Tagged , , , | 3 Comments

Modulating laser intensity on multiple timescales (x, y and z)

Posted on March 25, 2016 by P.T.R. Rupprecht

In point-scanning microscopy and especially when using resonant scanners, the intensity of the beam is typically modulated using a Pockels cell. For resonant scanning, the dwell time per micrometer is not constant along the scanned line, and one wants either to … Continue reading →

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Undistort/unwarp images for resonant scanning microscopy

Posted on February 16, 2016 by P.T.R. Rupprecht

For image acquisition using a resonant scanning microscope, one of the image axes is scanned non-linearly, following the natural sinusoidal movements of the resonant scanner. This leads to a distortion of the acquired images, unless a online correction algorithm or … Continue reading →

Posted in Calcium Imaging, Microscopy | Tagged , , , , | 1 Comment

Some interesting 2-photon microscopy papers

Posted on March 13, 2014 by P.T.R. Rupprecht

In the last few months, I built a special kind of 2P-microsope. In the meantime, I encountered some papers on microscope techniques which I found interesting and worth a side-note. Using AODs instead of galvoscanners for point-scanning: High-speed in vivo … Continue reading →

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