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Category Archives: Calcium Imaging
Interesting papers on online motion correction for calcium imaging
In a living animal, the brain is moving up and down in the skull. This brain motion can be due to breathing, heartbeat, tongue movements, but also due to changes of posture or running. For each brain region and posture, … Continue reading
Posted in Calcium Imaging, Data analysis, Imaging, machine learning, Microscopy, Neuronal activity, neuroscience
Tagged Calcium Imaging, Data analysis, Microscopy, photons, Scanning
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Ambizione fellowship and an open PhD position
I’m glad to share that I am going to start my own junior research group at the University of Zurich in March 2023! As an Ambizione fellow, I will receive funding for my own salary, some equipment, consumables and a … Continue reading
Self-supervised denoising of calcium imaging data
This blog post is about algorithms based on deep networks to denoise raw calcium imaging movies. More specifically, I will write about the difficulties to interprete their outputs, and on how to address these limitations in future work. I will … Continue reading
Video introduction to CASCADE
A video with tutorial on CASCADE, a supervised method to infer spike rates from calcium imaging data. Continue reading
Simple geometrical optics to understand and design point-scanning microscopes
Custom-built microscopes have become more and more sophisticated over the last years to provide a larger FOV, better resolution through some flavor of adaptive optics or simply more neurons simultaneously. Professional optical engineers are hired to design the ideal lens … Continue reading
Public peer review files
Peer-review is probably the most obscure part of the publication of scientific results. In this blog post, I would like to make the point that the best way to learn about it – except by being directly involved – is … Continue reading
Posted in Calcium Imaging, Imaging, machine learning, Microscopy, Neuronal activity, Review
Tagged deep learning, Microscopy, neuroscience, peer review
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Large-scale calcium imaging & noise levels
Calcium imaging based on two-photon scanning microscopy is a standard method to record the activity of neurons in the living brain. Due to the point-scanning approach, sampling speed is limited and the dwell time on a single neuron reduces with … Continue reading
Posted in Calcium Imaging, Data analysis, Imaging, Microscopy, Neuronal activity
Tagged Calcium Imaging, Data analysis, Microscopy, photons, Scanning
6 Comments
5 reasons why to use Cascade for spike inference
Our paper on A database and deep learning toolbox for noise-optimized, generalized spike inference from calcium imaging is out now in Nature Neuroscience. It consists of a large and diverse ground truth database with simultaneous calcium imaging and juxtacellular recordings … Continue reading
Fast scanning, triplet states and photon yield
In point-scanning microscopy like two-photon or confocal microscopy, a focused laser beam is scanned across the field of view and thereby sequentially recovers an image of the object. In this blog post, I will discuss the idea that scanning faster … Continue reading
Posted in Calcium Imaging, Imaging, Microscopy, Neuronal activity
Tagged Calcium Imaging, Microscopy, photons, Scanning, zebrafish
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Online spike rate inference with Cascade
To infer spike rates from calcium imaging data for a time point t, knowledge about the calcium signal both before and after time t is required. Our algorithm Cascade (Github) uses by default a window that is symmetric in time … Continue reading
A blog about neurophysiology 