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Tag Archives: zebrafish
The crow as an animal model for neuroscience
Close to my apartment in the outskirts of Basel, green fields and some small woods lie basically in front of my house door. This is also where some flocks of crows gather around, partly searching the fields for food, partly … Continue reading
Posted in electrophysiology, Imaging, Neuronal activity, Uncategorized
Tagged consciousness, electrophysiology, zebrafish
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Whole-cell patch clamp, part 1: introductory reading
Ever since I my interested in neuroscience become more serious, I was fascinated by the patch clamp technique, especially applied for the whole cell. Calcium imaging or multi-channel electrophysiology (recent review) is the way to go in order to get an idea what … Continue reading
Posted in electrophysiology, Microscopy, zebrafish
Tagged Calcium Imaging, electrophysiology, patch clamp, zebrafish
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The larval zebrafish, and the adult zebrafish
Zebrafish are often used as a model organism for in vivo brain imaging, because they are transparent. Or at least that is what many people think who do not work with zebrafish. In reality, most people use zebrafish larvae for in vivo … Continue reading
Posted in Calcium Imaging, Neuronal activity, zebrafish
Tagged Calcium Imaging, zebrafish
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Spatial visualization of temporal components for neuronal activity imaging
The standard analysis workflow for neuronal activity imaging based on calcium signals is to 1) draw ROIs around putative neurons, 2) extract the average fluorescence time trace of this ROI, 3) work with this timetrace for subsequent analysis (principal components, … Continue reading
Posted in Calcium Imaging, Data analysis, Imaging, Neuronal activity
Tagged Calcium Imaging, zebrafish
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Undistort/unwarp images for resonant scanning microscopy
For image acquisition using a resonant scanning microscope, one of the image axes is scanned non-linearly, following the natural sinusoidal movements of the resonant scanner. This leads to a distortion of the acquired images, unless a online correction algorithm or … Continue reading
Posted in Calcium Imaging, Microscopy
Tagged Calcium Imaging, laser scanning microscopy, Microscopy, Scanning, zebrafish
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PhD at the FMI
In April, I started my PhD in neuroscience in the Friedrich lab at the FMI. Topic will be the investigation of brain areas for higher olfactory processing in the zebrafish, and I’ll be working with different physiological methods.
A blog about neurophysiology 