Category Archives: Neuronal activity

Spatial visualization of temporal components for neuronal activity imaging

Posted on May 3, 2016 by P.T.R. Rupprecht

The standard analysis workflow for neuronal activity imaging based on calcium signals is to 1) draw ROIs around putative neurons, 2) extract the average fluorescence time trace of this ROI, 3) work with this timetrace for subsequent analysis (principal components, … Continue reading →

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Fast z-scanning using a voice coil motor

Posted on April 6, 2016 by P.T.R. Rupprecht

We just published a paper on fast remote z-scanning using a voice coil motor. For 2P calcium imaging. It’s a nice paper with some interesting technical details. The starting point was the remote z-scanning scheme used by Botcherby et al. (2012) from … Continue reading →

Posted in Calcium Imaging, Imaging, Microscopy, Neuronal activity | Tagged , , , | 3 Comments

Beyond correlation analysis

Posted on February 8, 2014 by P.T.R. Rupprecht

There are many papers out that use calcium imaging of a lot (tens to thousands) of neurons in animal brains. When I first encountered these kind of publications (roughly a year ago), it took me some hours to become familiar … Continue reading →

Posted in Calcium Imaging, Data analysis, Neuronal activity | 2 Comments