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Author Archives: P.T.R. Rupprecht
Point spread functions
One way to characterize the quality of one’s microscope is to measure the point spread function (PSF), that is the image that is created by a point source (which can be a fluorescent bead smaller than the expected size of the … Continue reading
Aphantasia
A couple of days ago, during a hiking tour close to Luzern, I met a medical doctor from Israel who decided to join me for my hike for the rest of the day. After some time, when I made fun of … Continue reading
EODs for kHz imaging
J. Schneider et al., and S. Hell recently published a paper on STED microscopy, using EODs (electro-optical deflectors) to scan 512 x 512 pixels at frame rates of 1000 Hz. Compared to AODs, EODs offer the costumer-friendly advantage of not dispersing … Continue reading
A pockels cell with a broken crystal
In a 2P microscope, pockels cells are employed for fast control of the laser beam intensity. I use it for both switching off the laser beam during turnarounds of the resonant scanner, between two frames if they are not immediately one after … Continue reading
Finding the engram
In their Nature Reviews|Neuroscience article, Finding the engram, Sheena Josselyn, Stefan Köhler and Paul Frankland discuss the recent developments, mainly in circuit neuroscience in mice, that contributed to finding memories on the cellular level, the so-called engram. They accumulate the evidence from … Continue reading
A simple non-graphical user interface in Matlab : keyboard callback functions
I’m not the first person to be annoyed by Matlabs guide (a tool used to generate GUIs that, unfortunately, are difficult to understand and painful to modify afterwards). Some months ago, I was looking for a way to implement a lightweight user interface for analyzing big … Continue reading
A network of pointers
When thinking about the way we think, it certainly makes sense to begin with a point which is also used by our thoughts as a starting point, which is sensual experience. To give an example, it is easy to imagine how a … Continue reading
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Tagged associative network, neuronal network, pointers, theoretical neuroscience
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The unsolved problems of neuroscience
The neuroskeptic blog recently mentioned a viewpoint paper which includes a list of the solved and unsolved problems of neuroscience. I’m probably not yet as deep into neuroscience as is the author of the paper, but I find it tempting to sharpen my … Continue reading
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Gain and photons per pixel
In 2010, Labrigger wrote about how to measure the gain of a imaging system. As mentioned there in the comments, this was discussed in more detail by James Pawley in the confocal microscopy mailing list quite recently (following a question I asked to the list), and I was … Continue reading
Colormaps (without colorspace theory)
The labrigger blog keeps posting links to all kind of colormaps, so I tried out some of them. Being partially colorblind, I do not like the default colormaps e.g. of Matlab. Here are some noisy data, with two different scalings … Continue reading
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A blog about neurophysiology 