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Tag Archives: Microscopy
Point spread functions
One way to characterize the quality of one’s microscope is to measure the point spread function (PSF), that is the image that is created by a point source (which can be a fluorescent bead smaller than the expected size of the … Continue reading
Gain and photons per pixel
In 2010, Labrigger wrote about how to measure the gain of a imaging system. As mentioned there in the comments, this was discussed in more detail by James Pawley in the confocal microscopy mailing list quite recently (following a question I asked to the list), and I was … Continue reading
Some interesting 2-photon microscopy papers
In the last few months, I built a special kind of 2P-microsope. In the meantime, I encountered some papers on microscope techniques which I found interesting and worth a side-note. Using AODs instead of galvoscanners for point-scanning: High-speed in vivo … Continue reading
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Tagged Calcium Imaging, Microscopy, Scanning, Temporal Focusing
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A blog about neurophysiology 