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Tag Archives: Calcium Imaging
Dirigo: a future Python alternative to Scanimage?
Over the last 20 years, many microscopes that are capable of resonant scanning two-photon microscopy have converged on using ScanImage, a powerful software package with many strengths but also two downsides: First, it is no longer open-source, and second, it … Continue reading
Posted in Calcium Imaging, Imaging, Microscopy, neuroscience
Tagged Calcium Imaging, Microscopy, photons, Scanning, technology
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Interesting papers on astrocyte ultrastructure and function
Astrocytes are brain cells that are often completely overlooked and dismissed or, in the opposite extreme, presented as mysterious devices that somehow solve all problems of computational neuroscience. The truth is somewhere in the middle, but it is not clear yet where exactly. First of all, we still do not understand even the basic mechanistic rules by which individual astrocytes operate. For neurons, we have precise, often even mathematical ideas how they receive input via synapses, how they depolarize, and how depolarizations propagate to the soma and elicit action potentials. For astrocytes, this mechanistic book of rules is still missing – and this gap limits our ability to interpret experiments or build coherent theories. Continue reading
Posted in Astrocytes, Calcium Imaging, Data analysis, Microscopy, neuroscience, Reviews
Tagged Calcium Imaging, Microscopy, neuroscience, science
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I never meant to study the brainstem
I was recently invited to contribute a guest post on Substack by Vijay Iyer – some years ago one of the main developers of ScanImage in the lab of Karel Svoboda, and more recently a strong advocate for ME/CFS awareness … Continue reading
Posted in Astrocytes, Brain machine interface, Calcium Imaging, hippocampus, Locus coeruleus, Microscopy, writing
Tagged arousal, brain, Calcium Imaging, Microscopy, neuroscience, photons, science, stress
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Interesting papers on the role of cell organelles of neuronal dendrites in calcium signaling
Researchers utilize calcium imaging to track neuronal activity in the brain, highlighting the role of calcium as a signaling molecule. Recent studies investigate intracellular calcium dynamics, particularly its coupling between organelles and its influence on synaptic plasticity in dendrites. Findings emphasize the necessity of in vivo methods for accurate insights into neuronal behavior. Continue reading
Posted in Calcium Imaging, Data analysis, hippocampus, Microscopy, neuroscience, Reviews
Tagged Calcium Imaging, Microscopy
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Interesting papers on VIP interneurons in cortex and hippocampus
Interneurons are inhibitory neurons in the brain that are thought to shape the computations performed by principal cells. The effects of inhibition can be rather diverse, depending on which neurons – or which parts of a neuron (dendrites, soma, or … Continue reading
Posted in Calcium Imaging, Data analysis, hippocampus, Imaging, Neuronal activity, neuroscience, Reviews
Tagged Calcium Imaging, Data analysis
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Accurately computing noise levels for calcium imaging data
It is fascinating how much data quality can vary between different calcium imaging data sets. In this blog post, I will discuss a metric to quantify and compare data quality and in particular shot noise between calcium imaging datasets. This … Continue reading
Open PhD position in my research group
Are you a finishing Master’s student with a quantitative background and are interested in neuroscience? This is your opportunity. Project: You will be supervised by Dr. Peter Rupprecht and Prof. Fritjof Helmchen at the Brain Research Institute, University of Zurich. … Continue reading
How to compute ΔF/F from calcium imaging data
Many neuroscientists use calcium imaging to record the activity from neurons (or other cells in the brain). The video below was recorded by Sian Duss and me in hippocampal CA1 pyramidal cells in mice. To make calcium imaging traces comparable … Continue reading
Posted in Calcium Imaging, Data analysis, hippocampus, Imaging, Microscopy, neuroscience
Tagged Calcium Imaging, Data analysis
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Online spike inference with GCaMP8
Calcium imaging is used to record the activity of neurons in living animals. Often, these activity patterns are analyzed after the experiments to investigate how the brain works. Alternatively, it is also possible to extract the activity patterns in real … Continue reading
Detecting single spikes from calcium imaging
There are two mutually exclusive holy grails of calcium imaging: First, recording from the highest number of neurons simultaneously. Second, detecting spike patterns with single-spike precision. This blog post focuses on the latter. Many studies have claimed to demonstrate single-spike … Continue reading
A blog about neurophysiology 