Monthly Archives: April 2025

Accurately computing noise levels for calcium imaging data

Posted on April 25, 2025 by P.T.R. Rupprecht

It is fascinating how much data quality can vary between different calcium imaging data sets. In this blog post, I will discuss a metric to quantify and compare data quality and in particular shot noise between calcium imaging datasets. This … Continue reading →

Posted in Calcium Imaging, Data analysis, electrophysiology, Imaging, machine learning, Neuronal activity, neuroscience | Tagged , , , , | Leave a comment

Open PhD position in my research group

Posted on April 3, 2025 by P.T.R. Rupprecht

Are you a finishing Master’s student with a quantitative background and are interested in neuroscience? This is your opportunity. Project: You will be supervised by Dr. Peter Rupprecht and Prof. Fritjof Helmchen at the Brain Research Institute, University of Zurich. … Continue reading →

Posted in Calcium Imaging, Data analysis, hippocampus, Imaging, machine learning, Microscopy, Neuronal activity, neuroscience | Tagged , , , , , | Leave a comment

How to compute ΔF/F from calcium imaging data

Posted on April 2, 2025 by P.T.R. Rupprecht

Many neuroscientists use calcium imaging to record the activity from neurons (or other cells in the brain). The video below was recorded by Sian Duss and me in hippocampal CA1 pyramidal cells in mice. To make calcium imaging traces comparable … Continue reading →

Posted in Calcium Imaging, Data analysis, hippocampus, Imaging, Microscopy, neuroscience | Tagged , | Leave a comment